Total Aflatoxin (AFT) ELISA Test Kit


【Specification】

96T/kit        96T*5/kit


【Principle of Test】

The Total Aflatoxin (AFT) ELISA Test Kit is based on a competitive colorimetric ELISA assay.

The toxin of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin and enzyme-conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The enzyme-conjugate, will bined with the primary antibody that is complexed to the toxin coated on the plate wells, last, take caloration with TMB substrate. The samples absorbance value is an inverse relationship with AFT content. Lastly, compared with the standard curve can be concluded the AFT residues in sample.


【Composition of the Kit】

Microelisa Stripplate, Standard , Enzyme conjugate, Antibody working solution , Concentrated Wash Solution 20×, Substrate A solution, Substrate B solution, Stop Solution.


【Technique Data】

1. Kit sensitivity: 0.02ppb (ng/ml)

2. Reactive mode: 25℃, 30min～15min

3. Detection Limits:

Sample

Detection Limits

Cereal

0.1 ppb

Compound feed

0.2ppb

Sauces, Wheat, Other feed

0.2ppb

Edible oil, peanut

0.2ppb

Beer

0.2 ppb

Wine, soy, vinegar

0.1 ppb

4. Cross-reaction rate:

Aflatoxin B1 …………………………………….……….100%

Aflatoxin B2 ………………………………..…………….75%

Aflatoxin G1 ……………………………..……………….100%

Aflatoxin G2 ……………………………………………….97%

Aflatoxin M1 ……………………………..……………….30%

5. Recovery rate:

Cereal, Compound feed ……………………………………. 85±15%

Peanut ……………………………………………………………... 82±15%

Edible oil …………………………………………………..……….. 85±15%

Sauces, Wheat, Other feed …………………………….….. 83±15%

Beer ………………………………………………………………….. 84±15%

Wine, soy, vinegar ……………………………….……….….. 87±15%   


【Storage Conditions】

1.The kit shall be stored at [2-8 ℃]. Expiry date: 12 months

2.The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.