Nitrofurantoin (AHD) ELISA Test Kit


【Specification】

96T/kit        96T*5/kit


【Principle of Test】

The Nitrofurantoin (AHD) ELISA Test Kit is using indirect competitive enzyme immunoassay method.

The Nitrofurantoin coupling antigen has been coated in the plate wells. During the detection, add Standards or samples to the well, AHD of samples will compete with coated AHD coupling antigen to combine with antibody, after adding antibody working solution, Horseradish Peroxidase (HRP), take coloration with TMB substrate. The samples A value is an inverse relationship with AHD residue content. Lastly, compared with the standard curve can be concluded the AHD residues in sample.


【Composition of the Kit】

Microelisa Stripplate, Standard , Enzyme conjugate, Antibody working solution , Concentrated Wash Solution 20×, concentrated Redissolving solution , Substrate A solution, Substrate B solution, Stop Solution, Derivatization reagent.


【Technique Data】

1. Kit sensitivity: 0.02ppb (ng/ml)

2. Reactive mode: 25℃, 45min～15min

3. Detection Limits:

Sample

Detection Limits

Tissue, Liver

0.04 ppb

Honey, Milk

0.04 ppb

Milk powder, Egg powder

0.04 ppb

Fish, Shrimp

0.06 ppb

4. Cross-reaction rate:

Name

Cross-reaction rate

AHD

100%

AMOZ

<0.1%

AOZ

<0.1%

SEM

<0.1%

5. Recovery rate:

Sample

Recovery rate

Tissue, Liver

80±25%

Honey, Milk, Prepared intestine

75±15%

Milk powder, Egg powder

85±25%


【Storage Conditions】

1.The kit shall be stored at [2-8 ℃]. Expiry date: 12 months

2.The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.