Aflatoxin albumin B1 (AFB1) ELISA Test Kit


【Specification】

96T/kit        96T*5/kit


【Principle of Test】

The Aflatoxin B1 (AFB1) ELISA Test Kit is based on a competitive colorimetric ELISA assay.

The toxin of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The second antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the toxin coated on the plate wells, last take coloration with TMB substrate. The samples absorbance value is an inverse relationship with AFB1 content. Lastly, compared with the standard curve can be concluded the AFB1 residues in sample.


【Composition of the Kit】

Microelisa Stripplate, Standard , Enzyme conjugate, Antibody working solution , Concentrated Wash Solution 20×, Substrate A solution, Substrate B solution, Stop Solution.


【Technique Data】

1. Kit sensitivity: 0.01ppb (ng/ml)

2. Reactive mode: 25℃, 30min～15min

3. Detection Limits:

Sample

Detection Limits

Cereals

0.05 ppb

Compound feed.

0.1 ppb

Edible oil, peanut

0.2 ppb

Sauces, Wheat, Other feed

0.1 ppb

Beer

0.1 ppb

Wine, soy, vinegar

0.05 ppb

4. Cross-reaction rate:

Aflatoxin B1………………………….100%

5. Recovery rate:

Sample

Recovery rate

Sample

Recovery rate

Cereals

85%±15%

Vinegar

87±15%

Compound feed.

85%±15%

Beer

84±15%

Peanut

82±15%

Wine

87±15%

Edible oil

85%±15%

Soy

87±15%

Sauces, Wheat,

83±15%

Other feed

83±15%


【Storage Conditions】

1.The kit shall be stored at [2-8 ℃]. Expiry date: 12 months

2.The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.