Chloramphenicol (CAP) ELISA Test Kit


【Specification】

96T/kit        96T*5/kit


【Principle of Test】

The Chloramphenicol (CAP) ELISA Test Kit is using indirect competitive enzyme immunoassay method.

The CAP coupling antigen has been coated in the plate wells. During the detection, add Standards or samples to the well, CAP of samples will compete with coated CAP coupling antigen to combine with antibody, after adding antibody working solution, Horseradish Peroxidase (HRP), take coloration with TMB substrate. The samples A value is an inverse relationship with CAP residue content. Lastly, compared with the standard curve can be concluded the CAP residues in sample.


【Composition of the Kit】

Microelisa Stripplate, Standard , Enzyme conjugate, Antibody working solution , Concentrated Wash Solution 20×, concentrated Redissolving solution , Substrate A solution, Substrate B solution, Stop Solution.


【Technique Data】

1. Kit sensitivity: 0.025 ppb (ng/ml)

2.Reactive mode: 25℃, 30min～30min～15min

3.Detection Limits:

Sample

Detection Limits

Tissue, Liver, Honey, Milk

0.0125 ppb

Eggs

0.05 ppb

Urine, Serum, Casing, Feed, Milk powder

0.025 ppb

4.Cross-reaction rate:

Name

Cross-reaction rate

Chloramphenicol

100%

Thiamphenicol

＜0.1%

Florfenicol

＜0.1%

5. Recovery rate:

Sample

Recovery rate

Tissue, Liver

85% ± 20% 

Honey, Casing

85% ± 25%

Milk, Feed

75% ± 25%

Urine, Serum

70% ± 20%

【Storage Conditions】

1.The kit shall be stored at [2-8 ℃]. Expiry date: 12 months

2.The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.