Clenbuterol (Cle) ELISA Test Kit


【Specification】

96T/kit        96T*5/kit


【Principle of Test】

The method of Clenbuterol (Cle) ELISA Test Kit is based on a competitive colorimetric ELISA assay.

The coupling antigen of Clenbuterol has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target antigen. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the coated antigen attached to the well. Then add secondary antibody, tagged with a Horseradish Peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells.

The resulting color intensity, after addition of substrates, has an inverse relationship with the Cle residues concentration in the sample.


【Composition of the Kit】

Microelisa Stripplate, Standard , Enzyme conjugate, Antibody working solution , Concentrated Wash Solution 20×, concentrated Redissolving solution , Substrate A solution, Substrate B solution, Stop Solution.


【Technique Data】

1. Kit sensitivity: 0.05 ppb (ng/ml)

2.Reactive mode: 25℃, 30min～15min

3.Detection Limits:

Sample

Detection Limits

Urine

0.05ppb

Tissue (method 1)

0.2ppb

Tissue (method 2)

0.05ppb

Feed

0.5PPB

4.Cross-reaction rate:

Name

Cross-reaction rate

Clenbuterol  

100%

Terbutaline

<1%

Mabuterol

<1%

Brombuterol

<1%

Salbutamol

<1%

Ractopamine

<1%

5. Recovery rate:

Sample

Recovery rate

Urine

95±10%

Tissue, Feed

85±15%


【Storage Conditions】

1.The kit shall be stored at [2-8 ℃]. Expiry date: 12 months

2.The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.