Ciprofloxacin (CPFX) ELISA Test Kit

【Specification】

96T/kit        96T*5/kit

【Principle of Test】

The Ciprofloxacin (CPFX) ELISA Test Kit is based on a competitive colorimetric ELISA assay.

The ciprofloxacin antibody has been coated in the plate wells. During the analysis, sample is added along with the ciprofloxacin-horseradish peroxidase (Ciprofloxacin-HRP) conjugate. If the ciprofloxacin residue is present in the sample, it will compete for the ciprofloxacin antibody, thereby preventing the Ciprofloxacin-HRP from binding to the antibody attached to the well, last, take coloration with TMB substrate. The samples A value is an inverse relationship with CPFX residue content. Lastly, compared with the standard curve can be concluded the CPFX residues in sample.

【Composition of the Kit】

Microelisa Stripplate, Standard , Enzyme conjugate, Antibody working solution , Concentrated Wash Solution 20×, concentrated Redissolving solution , Substrate A solution, Substrate B solution, Stop Solution.

【Technique Data】

1. Kit sensitivity: 0.1 ppb (ng/ml)

2.Reactive mode: 25℃, 45min～15min

3.Detection Limits:

Sample	Detection Limits
Tissue(chicken, pork, fish, shrimp)	0.3ppb
Honey	0.4ppb
Milk	3ppb
Milk powder	6ppb
Egg	3ppb

4.Cross-reaction rate:

Name	Cross-reaction rate
Ciprofloxacin	100%
Oxolin acid	28%
Levofloxacin	10%
Lomefloxacin	4%
Marbofloxacin	4%
Sarafloxacin	110%

5. Recovery rate:

Tissue, honey, eggs, milk, milk powder ……………85±15%

【Storage Conditions】

1.The kit shall be stored at [2-8 ℃]. Expiry date: 12 months

2.The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.

months.