Deoxynivalenol (DON) ELISA Test Kit

【Specification】

96T/kit        96T*5/kit

【Principle of Test】

The Deoxynivalenol (DON) ELISA Test Kit is based on a competitive colorimetric ELISA assay.

The DON antigen has been coated in the plate wells. During the analysis, sample is added along with the DON-horseradish peroxidase (DON-HRP) conjugate. If the DON residue is present in the sample, it will compete for the DON antibody, thereby preventing the DON-HRP from binding to the antibody attached to the well, last, take coloration with TMB substrate. The samples absorbance value is an inverse relationship with DON content. Lastly, compared with the standard curve can be concluded the DON residues in sample.

【Composition of the Kit】

Microelisa Stripplate, Standard , Enzyme conjugate, Antibody working solution , Concentrated Wash Solution 20×, concentrated Redissolving solution , Substrate A solution, Substrate B solution, Stop Solution.

【Technique Data】

1. Kit sensitivity: 3ppb (ng/ml)

2. Reactive mode: 37℃, 30min～30min～15min

3. Detection Limits:

Sample

Detection Limits

Cereals

150 ppb

Feed.

150 ppb

4. Cross-reaction rate:

Deoxynivalenol ………………………….100%

3- acetyl-DON ………………………….. <1%

5. Recovery rate:

Cereal …………………………………..……. 85±15%

Compound feed …………………………………….. 85±15%

【Storage Conditions】

1.The kit shall be stored at [2-8 ℃]. Expiry date: 12 months

2.The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.