Enrofloxacin (ENR) ELISA Test Kit


【Specification】

96T/kit        96T*5/kit


【Principle of Test】

The Enrofloxacin (ENR) ELISA Test Kit is based on a competitive colorimetric ELISA assay.

The Enrofloxacin antibody has been coated in the plate wells. During the analysis, sample is added along with the Enrofloxacin-horseradish peroxidase (Enrofloxacin -HRP) conjugate. If the Enrofloxacin residue is present in the sample, it will compete for the Enrofloxacin antibody, thereby preventing the Enrofloxacin-HRP from binding to the antibody attached to the well, last, take coloration with TMB substrate. The samples A value is an inverse relationship with ENR residue content. Lastly, compared with the standard curve can be concluded the ENR residues in sample.


【Composition of the Kit】

Microelisa Stripplate, Standard , Enzyme conjugate, Antibody working solution , Concentrated Wash Solution 20×, concentrated Redissolving solution , Substrate A solution, Substrate B solution, Stop Solution.


【Technique Data】

1. Kit sensitivity: 0.1 ppb (ng/ml)

2.Reactive mode: 25℃, 45min～15min

3.Detection Limits:

Sample

Detection Limits

Tissue(chicken, pork, fish, shrimp)

0.3ppb

Honey

0.4ppb

Milk

3ppb

Milk powder

6ppb

Egg

3ppb

4.Cross-reaction rate:

Name

Cross-reaction rate

Enrofloxacin

100%

Oxolin acid

28%

Levofloxacin

10%

Lomefloxacin

4%

Marbofloxacin

4%

Sarafloxacin

110%

5. Recovery rate:

Tissue, honey, eggs, milk, milk powder ……………85±15%


【Storage Conditions】

1.The kit shall be stored at [2-8 ℃]. Expiry date: 12 months

2.The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.