Fluoroquinolones (QNS) ELISA Test Kit


【Specification】

96T/kit        96T*5/kit


【Principle of Test】

The Fluoroquinolones (QNS) ELISA Test Kit is using indirect competitive enzyme immunoassay method.

The QNS coupling antigen has been coated in the plate wells. During the detection, add Standards or samples to the well, QNS of samples will compete with coated FQNS coupling antigen to combine with antibody, after adding antibody working solution, Horseradish Peroxidase (HRP), take coloration with TMB substrate. The samples A value is an inverse relationship with QNS residue content. Lastly, compared with the standard curve can be concluded the QNS residues in sample.

【Composition of the Kit】

Microelisa Stripplate, Standard , Enzyme conjugate, Antibody working solution , Concentrated Wash Solution 20×, concentrated Redissolving solution , Substrate A solution, Substrate B solution, Stop Solution.


【Technique Data】

1. Kit sensitivity: 0.1 ppb (ng/ml)

2.Reactive mode: 25℃, 45min～15min

3.Detection Limits:

Sample

Detection Limits

Tissue(chicken, pork, fish, shrimp)

0.3ppb

Honey

0.4ppb

Milk

3ppb

Milk powder

6ppb

Egg

3ppb

4.Cross-reaction rate:

Name

Cross-reaction rate

Name

Cross-reaction rate

Enrofloxacin

100%

Ciprofloxacin

110%

Norfloxacin

100%

Enoxacin

66%

Difloxacin

84%

Ofloxacin(despinner)

58%

Flumequine

126%

Levofloxacin

10%

Sarafloxacin

107%

Oxolinic acid

28%

Danofloxacin

110%

Lomefloxacin

4%

Pefloxacin

146%

Marbofloxacin

4%

5. Recovery rate:

Tissue, honey, eggs, milk, milk powder ……………85±15%


【Storage Conditions】

1.The kit shall be stored at [2-8 ℃]. Expiry date: 12 months

2.The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.