Infectious Bronchitis Virus Antibody Test Kit



Specification

96T/kit    96T*2/kit   96T*5/kit


Principle of Test

The kit is for the qualitative determination of IBV ab in the sample, adopt IBV antigen to coat microtiter plate, make solid-phase antigen, then pipette samples to the wells, with anti- IBV ab conjugated Horseradish Peroxidase (HRP). Wash and remove non-combinative antibody and other components. Antibodies specific for the antigen will bind to the pre-coated antigen. After washing completely, add TMB substrate solution and color develops according to the amount of IBV ab. Reaction is terminated by the addition of a stop solution and the intensity of the color is measured at a wavelength of 450 nm. Compared with the CUTOFF value to judge if IBV ab exists in the sample or not.


Composition of the Kit

Microelisa Stripplate、HRP-Conjugate Reagent、Sample Diluent、Concentrated Wash Solution 20×、

Chromogen Solution A、Chromogen Solution B、Positive control、Negative control

Storage Conditions

1. The kit shall be stored at [2-8 ℃].

2. The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.

Instrument

Mircoplate reader (contain: 450nm, 630nm wavelength), thermostatic equipment (37 degrees Celsius), adjustable Pipette.