Melamine (Mel) ELISA Test Kit


【Specification】

96T/kit        96T*5/kit


【Principle of Test】

The Melamine (Mel) ELISA Test Kit is using indirect competitive enzyme immunoassay method.

The Melamine coupling antigen has been coated in the plate wells. During the detection, add Standards or samples to the well, MEL of samples will compete with coated MEL coupling antigen to combine with anti Mel antibody, after adding antibody working solution, Horseradish Peroxidase (HRP), take coloration with TMB substrate. The samples A value is an inverse relationship with MEL residue content. Lastly, compared with the standard curve can be concluded the MEL residues in sample.


【Composition of the Kit】

Microelisa Stripplate, Standard , Enzyme conjugate, Antibody working solution , Concentrated Wash Solution 20×, concentrated Redissolving solution , Substrate A solution, Substrate B solution, Stop Solution.


【Technique Data】

1. Kit sensitivity: 1 ppb (ng/ml)

2.Reactive mode: 25℃, 30min～15min

3.Detection Limits:

Sample

Detection Limits

Milk powder

20 ppb

Milk

27 ppb

Milk powder / Milk

1 ppb

Tissue（Chicken/pork/duck/fish/shrimp/liver）

2 ppb

Feed

100 ppb

Egg

20 ppb

Serum

4 ppb

4.Cross-reaction rate:

Name

Cross-reaction rate

Melamine  

100%

triazinetriol

60%

Triazin , Triazine diammonium

<1%

5. Recovery rate:

Sample

Recovery rate

Milk powder , Milk

90±20%

Tissue

85±20%

Feed

85±20%

Egg

80±20%


【Storage Conditions】

1.The kit shall be stored at [2-8 ℃]. Expiry date: 12 months

2.The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.