The test kit allows for the determination of Newcastle Disease Virus Antibody expression in poultry serum, plasma, egg yolk of chickens, ducks, goose, can be used to Newcastle Disease Virus vaccine immunization effect assessment.
Newcastle
Disease Virus Antibody Test Kit (Liquid blocking method)
【Specification】
96T/kit 96T*2/kit 96T*5/kit
【Principle of Test】
The kit, base on Liquid Blocking method, is for the qualitative
determination of NDV-ab in the sample, adopt NDV antigen to coat microtiter
plate, make solid-phase antigen. When experimenting, pipette the diluted
samples and antibody working solution into the wells. If there are NDV-ab in
samples, it will prevent the antibody solution which will combined with antigen
from wells. After washing completely, add TMB substrate solution and color
develops according to the amount of AIV-ab. Reaction is terminated by the
addition of a stop solution and the intensity of the color is measured at a
wavelength of 450 nm. Compared with the CUTOFF value to judge if NDV ab exists
in the sample or not.
【Composition of the Kit】
Microelisa Stripplate、HRP-Conjugate Reagent、Antibody working solution、Concentrated Wash
Solution 20×、?Chromogen Solution A、Chromogen
Solution B、Positive control、Negative
control
【Storage Conditions】
1. The kit shall be stored at [2-8 ℃].
2. The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.
【Instrument】
Mircoplate reader (contain: 450nm, 630nm wavelength),
thermostatic equipment (37 degrees Celsius), adjustable Pipette.
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