Newcastle Disease Virus Antibody Test Kit (Liquid blocking method)


 Specification

96T/kit    96T*2/kit   96T*5/kit

 

Principle of Test

The kit, base on Liquid Blocking method, is for the qualitative determination of NDV-ab in the sample, adopt NDV antigen to coat microtiter plate, make solid-phase antigen. When experimenting, pipette the diluted samples and antibody working solution into the wells. If there are NDV-ab in samples, it will prevent the antibody solution which will combined with antigen from wells. After washing completely, add TMB substrate solution and color develops according to the amount of AIV-ab. Reaction is terminated by the addition of a stop solution and the intensity of the color is measured at a wavelength of 450 nm. Compared with the CUTOFF value to judge if NDV ab exists in the sample or not.

 

Composition of the Kit

Microelisa Stripplate、HRP-Conjugate Reagent、Antibody working solution、Concentrated Wash Solution 20×、?Chromogen Solution A、Chromogen Solution B、Positive control、Negative control

 

Storage Conditions

1. The kit shall be stored at [2-8 ℃].

2. The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.

 

Instrument

Mircoplate reader (contain: 450nm, 630nm wavelength), thermostatic equipment (37 degrees Celsius), adjustable Pipette.