The test kit allows for the determination of Porcine Pseudorabies virus gE antibody (PRV-gE Ab) expression in Porcine serum and plasma, Can be used for the difference between clinical infected field virus pigs, and immuned pigs with vaccine of non gE gene.
Porcine Pseudorabies Virus gE Antibody Test Kit
【Specification】
96T/kit 96T*2/kit 96T*5/kit
【Principle of Test】
The kit is for the qualitative determination of PRV -gE ab in the sample, adopt PRV recombination gE protein antigen to coat microtiter plate, make solid-phase antigen, then pipette samples to the wells, with anti- Porcine PRV -gE ab conjugated Horseradish Peroxidase (HRP). Wash and remove non-combinative antibody and other components. Antibodies specific for the antigen will bind to the pre-coated antigen. After washing completely, add TMB substrate solution and color develops according to the amount of PRV -gE ab Reaction is terminated by the addition of a stop solution and the intensity of the color is measured at a wavelength of 450 nm. Compared with the CUTOFF value to judge if PRV -gE ab exists in the sample or not.
【Composition of the Kit】
Microelisa Stripplate、HRP-Conjugate Reagent、Sample Diluent、Concentrated Wash Solution 20×、
Chromogen Solution A、Chromogen Solution B、Stop Solution、Positive control、Negative control
【Storage Conditions】
1. The kit shall be stored at [2-8 ℃].
2. The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.
【Instrument】
Mircoplate reader (contain: 450nm, 630nm wavelength), thermostatic equipment (37 degrees Celsius), adjustable Pipette.
AIV-Ag test strip can be used for the farm and testing agency to test different subtype antigen
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