Sulfonamides (SAs) ELISA Test Kit


【Specification】

96T/kit        96T*5/kit


【Principle of Test】

The Sulfonamides (SAs) ELISA Test Kit is based on a competitive colorimetric ELISA assay.

The plate wells are coated with capture antibodies directed against anti- sulfonamide antibodies. Sulfonamide standards or sample solution, sulfonamide–HRP conjugate and anti-sulfonamide antibodies are added. Free sulfonamide and sulfonamide-HRP Conjugate compete for the sulfonamide antibody binding sites. At the same time, the anti- sulfonamide antibodies are also bound by the immobilized capture antibodies. Any unbound sulfonamide-HRP Conjugate is then removed in a washing step. Take coloration with TMB substrate. The samples A value is an inverse relationship with SAs residue content. Lastly, compared with the standard curve can be concluded the SAs residues in sample.

【Composition of the Kit】

Microelisa Stripplate, Standard , Enzyme conjugate, Antibody working solution , Concentrated Wash Solution 20×, concentrated Redissolving solution , Substrate A solution, Substrate B solution, Stop Solution.

【Technique Data】

1. Kit sensitivity: 0.5ppb (ng/ml)

2.Reactive mode: 25℃, 45min～15min

3.Detection Limits:

Sample

Detection Limits

Tissue ( High detection limit method )

0.5 ppb

Tissue ( Low detection limit method )

2.5 ppb

Urine, Serum

2 ppb

Honey

0.5 ppb

Milk

10 ppb

4.Cross-reaction rate:

Name

Cross-reaction rate

Tissue, Honey

95 ± 25%  

Urine, Milk, Serum

85 ± 25%

5. Recovery rate:

Name

Cross rate

Sensitivity ppb

Sulfamerazine (SM2)

100%

0.5

Sulfamonomethoxine (SMM)

670%

0.07

Sulfamethoxydiazine (SMD)

582%

0.09

Sulphormethoxine (SDM’)

451%

0.1

Sulfamerazine (SM1)

313 %

0.15

Sulfadiazine (SD or SDZ)

308 %

0.15

Sulfisomidine (SM2’)

241%

0.2

Sulfadimethoxine (SDM)

175%

0.3

Sulfamethizole (SMT)

165%

0.3

Sulfachlorpyrazine (Esb3)

67 %

0.8

Sulfathiazole (ST)

58%

0.9

Sulfachloropyridazine (SCPA)

58%

0.9

Sulfamethoxypyridazine (SMP)

57%

0.9

Sulfaquinoxaline (SQX)

42%

1

Sulfisoxazole (SIZ)

18 %

3

Sulfamethoxazole (SMZ)

18%

3


【Storage Conditions】

1.The kit shall be stored at [2-8 ℃]. Expiry date: 12 months

2.The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.