Tetracyclines (TCs) ELISA Test Kit


【Specification】

96T/kit        96T*5/kit


【Principle of Test】

The Tetracyclines (TCs) ELISA Test Kit is based on indirect competitive enzyme immunoassay method.

The Tetracyclines (TCs) coupling antigen has been coated in the plate wells. During the detection, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a Horseradish peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. Take coloration with TMB substrate. The samples A value is an inverse relationship with TCs residue content. Lastly, compared with the standard curve can be concluded the TCs residues in sample.


【Composition of the Kit】

Microelisa Stripplate, Standard , Enzyme conjugate, Antibody working solution , Concentrated Wash Solution 20×, concentrated Redissolving solution , Substrate A solution, Substrate B solution, Stop Solution.


【Technique Data】

1. Kit sensitivity: 0.05ppb (ng/ml)

2.Reactive mode: 37℃, 30min～30min～15min

3.Detection Limits:

Sample

Detection Limits

Tissue, Liver, Eggs

0.3ppb

Honey

2 ppb

Urine

0.5 ppb

4.Cross-reaction rate:

Name

Cross-reaction rate

Tetracyclines

100%

Minocycline

125%

Rolitetracycline

110%

Chlortetracycline

100%

Demeclocycline

35 %

Oxytetracycline

58 %

Doxycycline

45 %

5. Recovery rate:

Name

Cross-reaction rate

Tissue, Liver, Egg

85% ± 20% 

Honey

75% ± 20%

Urine

80% ± 20%


【Storage Conditions】

1.The kit shall be stored at [2-8 ℃]. Expiry date: 12 months

2.The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.